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EC Number
Purification (Commentary)
ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography
DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase
native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration; native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
Ni-nitrilotriacetic acid agarose colum
Ni-NTA agarose column
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant enzyme 0.7fold from Pichia pastoris cell culture medium by cation exchange chromatography and gel filtration
recombinant enzyme expressed in Pichia pastoris
recombinant expression of His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography
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