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Search Purification (Commentary)

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EC Number
expressed in Escherichia coli
near homogeneity
recombinant enzyme 1.2fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, dialysis, and ultrafiltration
recombinant enzyme, the expression and purification system has made the industrial production of the enzyme possible
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant N-terminally His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli by nickel affinity chromatography, anion exchange chromatography, and gel filtration
recombinant protein purified to 90%
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