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Search Purification (Commentary)

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EC Number
ammonium sulfate precipitation, hydroxyapatite column chromatography, Hi Trap Q column chromatography, and Superdex 200 gel filtration
centrifugation at 150000 xg, followed by succesive chromatography of the supernatant on DEAE-cellulose, Sephacryl S-300 and Heparin-Toyopearl
recombinant C-terminally His6-tagged SecS from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography and gel filtration to over 95% purity
recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration
Results 1 - 5 of 5