EC Number   |
|---|
   2.7.7.87 | attempts to purify the native forms of YdiC and YdiE by specific cleavage of the fusion proteins with thrombin or Factor Xa protease lead to multiple products, perhaps because the proteins are not completely stable in the cleavage reaction conditions |
| 2.7.7.87 | by Ni-NTA affinity chromatography to a purity of more than 95% by SDS-PAGE |
| 2.7.7.87 | gravity-flow chromatography on an Ni-NTA resin column, not associated with tRNAs when purified |
| 2.7.7.87 | gravity-flow chromatography on an Ni-NTA resin column, PaKEOPS complex co-purified with nucleic acids |
| 2.7.7.87 | Ni-NTA affinity chromatography |
| 2.7.7.87 | yrdC protein purified by Ni-NTA affinity chromatography, dialyzed against phosphate-buffered saline and concentrated using a YM-3 Centriprep filter |
