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Search Purification (Commentary)

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EC Number
Commentary
Reference
from encysting and non-encysting cells
full length and truncated enzymes using His-tag
glutathione Sepharose column chromatography and GSTrap column chromatography
glutathione Sepharose column chromatography and Superdex 75 gel filtration
GST fusion protein from E. coli
His-tag used for purifcation
partial
recombinant enzyme
recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and gel filtration, the His-tag is cleaved off by His-tagged MBP-3C protease and removed by affinity chromatography together with the protease; recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and gel filtration, the His-tag is cleaved off by His-tagged MBP-3C protease and removed by affinity chromatography together with the protease
Results 1 - 10 of 17 > >>