   2.7.1.227 | detergents Triton X-100, Nonidet P-40, Brij, Tween20, Tween 80 and octylglucoside all inactivate the enzyme. At moderate concentrations, the activity of the Triton X-100- and octylglucoside-solubilized material can be partially restored by inclusion of 5 mM phosphatidylinositol in the solubilization buffer. Detergents Empigen BB, N-dodecyl-N,N-(dimethylammonio)butanoate, Zwittergent 3-10, and amidosulfobetaine-16 all inactivate the enzyme essentially irreversibly. IPC synthase activity in membranes remaines largely intact and sedimentable at CHAPS concentrations of 4% where more than 90% of the phospholipids and 60% of the total proteins are extracted from the membranes |
