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Search Purification (Commentary)

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Results 1 - 5 of 5
EC Number
Commentary
Reference
native enzyme by heat treatment at 50C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
recombinant His-tagged enzyme from Mycobacterium smegmatis by nickel affinity chromatography
recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography
Results 1 - 5 of 5