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Search Purification (Commentary)

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EC Number
Commentary
Reference
Bio-Spin 6 column chromatography
enzyme is purified to at least 98% homogeneity with 42% final yield
Ni-NTA column chromatography
Ni2+-IDA agarose affinity chromatography and HiTrap desalting column chromatography
Ni2+-IDA agarose chromatography
Ni2+-IDA agarose chromatography and Superdex 200 gel filtration
partial purification by immobilized Ni2+ affinity column chromatography
Q-Sepharose fast flow column chromatography and Superdex 200 Hiload gel filtration
Q-Sepharose fast flow column chromatography and Superdex 200 HR 26/60 Hiload gel filtration
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