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Search Purification (Commentary)

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Results 1 - 4 of 4
EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 1.8.7.3- 743210
Display the reaction diagram Show all sequences 1.8.7.3phenyl-Sepharose column chromatography, and Superdex 200 gel filtration 744307
Display the reaction diagram Show all sequences 1.8.7.3purification of a massive (megadalton) complex of the electron-bifurcating Fdh-Hdr together with the CO2-reducing Fmd (Fdh-Hdr-Fmd hexamer), as well as of dimeric Fdh-Hdr-Fmd and Fdh-Hdr subcomplexes by anion exchange chromatography and gel filtration. Mass spectrometry reveals that the Fdh-Hdr-Fmd complex elutes from gel filtration primarily as a 1-MDa dimer of subunits FdhAB-MvhD-HdrABCFmdABCDFG. The Fdh-Hdr complexes are isolated as two oligomeric states, which contain FdhAB-MvhD-HdrABCFmdF. Two of five isoenzymes of FdhA and are detected in all complexes 765833
Display the reaction diagram Show all sequences 1.8.7.3Q-Sepharose column chromatography and Superdex 200 gel filtration 742382
Results 1 - 4 of 4