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EC Number
Purification (Commentary)
Reference
1.8.7.3
-
743210
1.8.7.3
phenyl-Sepharose column chromatography, and Superdex 200 gel filtration
744307
1.8.7.3
purification of a massive (megadalton) complex of the electron-bifurcating Fdh-Hdr together with the CO2-reducing Fmd (Fdh-Hdr-Fmd hexamer), as well as of dimeric Fdh-Hdr-Fmd and Fdh-Hdr subcomplexes by anion exchange chromatography and gel filtration. Mass spectrometry reveals that the Fdh-Hdr-Fmd complex elutes from gel filtration primarily as a 1-MDa dimer of subunits FdhAB-MvhD-HdrABCFmdABCDFG. The Fdh-Hdr complexes are isolated as two oligomeric states, which contain FdhAB-MvhD-HdrABCFmdF. Two of five isoenzymes of FdhA and are detected in all complexes
765833
1.8.7.3
Q-Sepharose column chromatography and Superdex 200 gel filtration
742382
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