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EC Number
Commentary
Reference
by a single chromatographic step under anaerobic conditions
by affinity chromatography
His-bind resin column chromatography
recombinant GST-tagged enzyme by glutathione affinity chromatography and ultrafiltration; recombinant HemN from Escherichia coli strain BL21(DE3)
recombinant HemN
recombinant His-tagged full-length enzyme and viperin (214-361) from Escherichia coli strain BL21 inclusion bodies, solubilization by 8 M urea and purification by nickel affinity chromatography and gel filtration
recombinant His-tagged HemN from Escherichia coli strain BL21(DE3) membranes by nickel affinity chromatography and gel filtration
recombinant terminally Strep-tagged HemNs from Escherichia coli to homogeneity
recombinant wild-type and mutant HemN
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