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EC Number
Purification (Commentary)
; native enzyme from the cytosolic fraction by gel filtration and immunoaffinity chromatography, recombinant His-tagged enzyme by nickel affinity chromatography to homogeneity and subsequent clevage of the tag by thrombin
; Orange-A column, reverse-affinity step, Mono Q column
; streptomycin precipitation, ion-exchange, hydroxylapatite
acid treatment, DE-52-, Matrex Orange A-, Matrex Blue A column chromatography
ammonium sulfate precipitation and Q-Sepharose column chromatography
by ion-exchange chromatography
centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase
ion-exchange, gel filtration, ammonium sulfate precipitation; preparative gel electrophoresis
Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration
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