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Search Purification (Commentary)

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EC Number
cells are centrifuged, sonicated, cell debris removed by centrifugation, membrane fraction separated from the soluble fraction by ultracentrifugation, chlorosome fractioned by sucrose density gradient (15-45%), for SDS-PAGE chlorosome fraction is incubated in methanol, centrifuged, the pellet containing the chlorosome envelop is subjected to SDS-PAGE, protein identification by MALDI-TOF and liquid chromatography-tandem mass spectrometry
partial, soluble fraction
partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants
Results 1 - 4 of 4