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EC Number
Purification (Commentary)
Reference
1.1.99.22
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287791
1.1.99.22
to homogeneity by Ni-affinity chromatography
668061
1.1.99.22
to homogeneity by anion exchange chromatography and Ni-affinity chromatography
674959
1.1.99.22
CM-Toyopearl C-650 column chromatography, and Sephadex G-200 gel filtration
740188
1.1.99.22
n-octyl-beta-D-thioglucoside, n-octyl-beta-D-glucoside, Mydol 10, and Mydol 12 are effective detergents to solubilize the enzyme
760731
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