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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and gel filtration, the His-tag is cleaved off by His-tagged MBP-3C protease and removed by affinity chromatography together with the protease 737391
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography 737096
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 760749, 761532
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis 737291
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage, and gel filtration 737319
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and ultrafiltration 755852
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His-tagged wild-type enzyme and mutant Y97N from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and ultrafiltration 760395
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 735518, 736517
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant N-terminally GST-tagged wild-type and mutant AGX1 from Escherichia coli strain (DE3) pLysS by glutathione affinity chromatography, the tag is cleaved off by PreScission protease, followed by gel filtration, and ultrafiltration 761091
Show all pathways known for 2.7.7.23Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.23recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 735569
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