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Search Purification (Commentary)

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EC Number
preparative gel electrophoresis
recombinant DHOD1, DHOD2 and DHOD3 with an N-terminal His6-tag, by affinity chromatography
recombinant His-tagged enzyme by nickel affinity chromatography
recombinant; to homogeneity by ion-exchange, hydrophobic interaction and gel filtration
soniciation and gel filtration
streptomycin sulfate and ammonium sulfate precipitation, DEAE-Sephacel-, affinity chromatography
the enzyme is fused with aspartate carbamoyltransferase as precursor to mature DHOD
to homogeneity by a single affinity chromatography step
to homogeneity by a single nickel affinity chromatography step
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