EC Number |
Posttranslational Modification |
Reference |
---|
3.2.1.57 | glycoprotein |
- |
136638 |
3.2.1.57 | glycoprotein |
highly N-glycosylated enzyme with 15 potential N-glycosylation sites (Asn-X-Ser/Thr), the side chain of a glycosylated asparagine residue is important for the stability of the enzyme, enzyme deglycosylation results in a decrease in thermostability. Enzyme deglycosylated with peptide-N-glycosidase F shows virtually no enzymatic activity, while a single GlcNAc residue remains following digestion with Endo H, and the activity of the Endo H-treated wild-type enzyme is 65% compared to intact, non-deglycosylated, wild-type enzyme |
-, 732013 |
3.2.1.57 | glycoprotein |
N-glycosylated |
136639 |
3.2.1.57 | glycoprotein |
recombinant IPU expressed in Pichia pastoris GS115 has a higher carbohydrate content, 41%, than native IPU, 12-15%, or recombinant IPU expressed in Aspergillus oryzae M-2-3, 34% |
655036 |
3.2.1.57 | glycoprotein |
the enzyme has 7 glycosylation sites |
-, 750801 |
3.2.1.57 | glycoprotein |
the sugar content of native IPU is 12.4-15.3%, that of recombinant IPU is 33.8%, deglycosylated enzyme by endoglycosidase H with a sugar content of 2.1% retains 65% of its original activity, kinetics of deglycosylated IPU, hybrid- and/or high-mannose types |
655805 |