Prunus dulcis | RNA is extracted from the tegument, nucellus and cotyledon of one sweet and two bitter (D05-187 and S3067) almond genotypes throughout fruit ripening. Sequences of nine positive prunasin hydrolases are obtained from all of the genotypes. Minor differences in the nucleotide and corresponding amino acid sequences are found between the nine prunasin hydrolases sequences. None of the differences can be related to the sweet or bitter flavour. Only PH691 from the D05-187 tegument shows reduced prunasin hydrolase activity; genotypes Lauranne (SkSk, sweet), D05-187 (sksk, bitter) and S3067 (sksk, bitter) |
751884 |