EC Number   |
Application |
Reference |
|---|
   7.2.2.14 | biotechnology |
Overexpression of enzyme in Escherichia coli results in formation of inclusion bodies. Co-expression of DnaK/DnaJ prevents inclusion bodies and leads to the integration of more enzyme into the membrane. Co-expression of GroEL/GroES, Ffh/4.5S-RNA or SecA are less effective |
670752 |
| 7.2.2.14 | medicine |
Mg2+ transport |
289130, 289131, 289132, 289133, 289135, 289136, 289137, 289138, 289139, 289140, 289141, 289142, 289143, 289145, 289146, 289147 |
| 7.2.2.14 | synthesis |
by overexpression of mgtA in Escherichia coli, a concentration of 32.41 g succinic acid per liter with a yield of 0.81 g per g glucose, can be obtained in a batch fermentation by using the low-cost mixture of Mg(OH)2 and NH3-H2O to replace MgCO3 as the alkaline neutralizer. The effect of the inhibitory compounds in lignocellulosic hydrolyzates on cell growth and succinic acid production can be relieved. In a 3-liter bioreactor, the overall productivity and yield of succinic acid in the whole anaerobic stage are 2.15 g per liter and h and 0.86 g per g total sugar, respectively |
733525 |
