EC Number   |
Application |
Reference |
|---|
   5.1.99.5 | synthesis |
enzymatic production of D-amino acids via a combination of hydantoin hydrolysis and hydantoin racemization. The D-forms of amino acids D-phenylalanine, D-tyrosine, D-tryptophan, O-benzyl-D-serine, D-valine, D-norvaline, D-leucine and D-norleucine can efficiently be converted from the corresponding DL-5-monosubtituted hydantoin compounds |
-, 669898 |
| 5.1.99.5 | synthesis |
reaction system for the production of D-amino acids from D,L-5-monosubstituted hydantoins based on recombinant Escherichia coli whole cell biocatalysts containing separately expressed D-hydantoinase, D-carbamoylase, and hydantoin recemase. The system shows high substrate specificity and is effective toward both aliphatic and aromatic D,L-5-monosubstituted hydantoins. At pH 8 and 50°C, 100% conversion of D,L-5-(2-methylthioethyl)-hydantoin (15 mM) into D-methionine is obtained in 30 min |
685769 |
| 5.1.99.5 | synthesis |
stereospecific production of L-amino acids from the corresponding DL-5-substituted hydantoins. DL-5-substituted hydantoins are converted exclusively to the L-forms of the corresponding N-carbamyl-amino acids by hydantoinase in combination with hydantoin racemase. The N-carbamyl-l-amino acids are then converted to L-amino acids by N-carbamyl-L-amino acid amidohydrolase |
-, 288947 |
