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4.4.1.29
synthesis
simultaneous expression of the phycocyanobilin-producing genes hox1 and pcyA of Arthrospira platensis with the phycobiliprotein gene cpcB and the lyase gene cpcS/U in Escherichia coli. Phycobiliprotein cpcB is successfully synthesized in Escherichia coli and co-expressed phycocyanobilins are attached though at a relatively low efficiency. The attachment of phycocyanobilins to phycobiliprotein cpcB is carried out mainly by co-expressed lyase cpcS/U but cpcB also shows some autocatalytic activity
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