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EC Number Application Commentary Reference
Display the reaction diagram Show all sequences 4.2.3.61analysis method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host provides for direct functional testing of the encoded gene product 666632
Display the reaction diagram Show all sequences 4.2.3.61synthesis construction of fusion proteins with farnesyl diphosphate synthase FPPS from Artemisia annua. The fusion enzymes produce epi-aristolochene from isopentenyl diphosphate through a coupled reaction. The Km values of FPPS and eAS for isopentenyl diphosphate and farnesyl diphosphate, respectively, are essentially the same for the single and fused enzymes. The bifunctional enzymes show a more efficient conversion of isopentenyl diphosphate to epi-aristolochene than the corresponding amount of single enzymes 711891
Display the reaction diagram Show all sequences 4.2.3.61synthesis expression system in Escherichia coli, highest expression level after 3 h induction with low concentration of IPTG and incubation at 27°C. Epiaristolochene synthase protein constitutes up to 35% of total Escherichia coli proteins 710990
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