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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.32synthesis increase of levopimaradiene synthesis in Escherichia coli by amplification of the flux toward isopentenyl diphosphate and dimethylallyl diphosphate precursors and reprogramming the rate-limiting downstream pathway by generating combinatorial mutations in geranylgeranyl diphosphate synthase and levopimaradiene synthase. The most productive pathway, combining precursor flux amplification and mutant synthases, confers approximately 2600fold increase in levopimaradiene levels. A maximum titer of approximately 700 mg/l is obtained by cultivation in a benchscale bioreactor 716753
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.32synthesis the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production, e.g. by abietadiene synthase, and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms, method overview 748548
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