EC Number   |
Application |
Reference |
|---|
    4.2.2.21 | analysis |
enzyme combined with chodroitin sulfatase ABC, EC 4.2.2.4, can be applied for the sequencing of chondroitin sulfate hexa- and octasaccharides with complex structures |
747033 |
| 4.2.2.21 | analysis |
micromethods for the measurement of as little as 3 pg of chondroitin sulfate A, B, or C in mixtures with other mucopolysaccharides or in urine |
-, 665449 |
| 4.2.2.21 | biotechnology |
preservation of ChABC activity during release by immobilizing ChABC in chitosan nerve conduits and encapsulating ChABC in poly(DL-lactic acid) microspheres using an appropriate stabilizer. Immobilizing ChABC in nerve conduitss markedly improves its stability. The activity of ChABC that is immobilized in chitosan nerve conduits by ionic bonding is 0.07 U/mg. 48% of this activity is retained at 48 h after immobilization. Poly(DL-lactic acid) microspheres, fabricated by the double emulsion method, are applied as carriers in the controlled release of ChABC. Stabilizers, including nanogold of 10 nm, polylysine of Mw 500-2000 and polylysine of Mw 20000-30000, are added to microspheres to maintain the activity of ChABC. Polylysine stabilizes ChABC most effectively. The ChABC activity is 0.0162 U/ml after seven days of release |
714652 |
| 4.2.2.21 | medicine |
preservation of ChABC activity during release by immobilizing ChABC in chitosan nerve conduits and encapsulating ChABC in poly(DL-lactic acid) microspheres using an appropriate stabilizer. Immobilizing ChABC in nerve conduitss markedly improves its stability. The activity of ChABC that is immobilized in chitosan nerve conduits by ionic bonding is 0.07 U/mg. 48% of this activity is retained at 48 h after immobilization. Poly(DL-lactic acid) microspheres, fabricated by the double emulsion method, are applied as carriers in the controlled release of ChABC. Stabilizers, including nanogold of 10 nm, polylysine of Mw 500-2000 and polylysine of Mw 20000-30000, are added to microspheres to maintain the activity of ChABC. Polylysine stabilizes ChABC most effectively. The ChABC activity is 0.0162 U/ml after seven days of release |
714652 |
| 4.2.2.21 | medicine |
sustained delivery of thermostabilized chABC enhances axonal sprouting and functional recovery after spinal cord injury. Animals treated with thermostabilized chABC in combination with sustained neurotrophin-3 delivery show significant improvement in locomotor function and enhanced growth of cholera toxin B subunit-positive sensory axons and sprouting of serotonergic fibers |
706560 |
| 4.2.2.21 | medicine |
the combination of cell transplantation with ChABC may be a potent therapeutic strategy for spinal cord injury |
705923 |
| 4.2.2.21 | synthesis |
Production of enzyme for industrial use. Induction of enzyme by addition of chondroitin sulfate C to growth medium. Optimal conditions are pH 8.0, 25°C, plus the addition of yeast extract, peptone and casamino acid. Induction is inhibited by chloramphenicol and actinomycin D |
34084 |
