EC Number   |
Application |
Reference |
|---|
   4.1.1.102 | brewing |
a positive relationship exists between single nucleotide polymorphisms in phenylacrylic acid decarboxylase PAD1 and ferulic acid decarboxylase FDC1 genes and the ferulic acid decarboxylation ability of industrial yeast strains. Sake, shochu, and standard top-fermenting yeasts contain a nonsense mutation of FDC1, whereas a frameshift mutation is identified in the FDC1 gene of bottom-fermenting yeast. No nonsense or frameshift mutations are detected in laboratory, wine, or weizen beer yeast strains. When FDC1 is introduced into sake and shochu yeast strains, the transformants exhibit ferulic acid decarboxylation activity |
734337 |
| 4.1.1.102 | degradation |
expression of aldehyde dehydrogenase Ald5, phenylacrylic acid decarboxylase Pad1, and alcohol acetyltransferases Atf1 and Atf2 increases conversion of coniferyl aldehyde, ferulic acid and p-coumaric acid. Combined overexpression of ALD5, PAD1, ATF1 and ATF2 helps Saccharomyces cerevisiae in phenolics conversion and tolerance |
-, 749347 |
| 4.1.1.102 | synthesis |
4-hydroxystilbene is synthesized from p-coumaric acid in four parallel continuous flow reactors, using a 3D printing process with agarose bioinks, and a subsequent palladium(II) acetate-catalysed Heck reaction, with a total yield of 14.7% on a milligram scale. The enzyme shows 38% residual activity after the printing process |
762925 |
| 4.1.1.102 | synthesis |
by expressing Rhodobacter sphaeroides tyrosine ammonia lyase, in Streptomyces mobaraense, which permits the synthesis of p-coumaric acid from glucose, a strain is obtained that produces high amounts of 4-vinylphenol |
747388 |
| 4.1.1.102 | synthesis |
for 4-vinylphenol production directly from cellulose, L-tyrosine ammonia lyase derived from Rhodobacter sphaeroides and phenolic acid decarboxylase from Streptomyces sviceus are introduced into endoglucanase-secreting Streptomyces lividans, and the 4-vinylphenol biosynthetic pathway is constructed therein. The created transformants successfully produce 4-vinylphenol directly from cellulose |
747296 |
| 4.1.1.102 | synthesis |
styrene production from biomass-derived carbon sources, by culture of Streptomyces lividans expressing FDC1 together with Streptomyces lividans/p-encP, which produces trans-cinnamic acid. The coculture system combined with the recovery of styrene using polystyrene resin beads XAD-4 allows the production of styrene from glucose, cellobiose, or xylooligosaccharides, respectively |
-, 748255 |
| 4.1.1.102 | synthesis |
synthesis of vanillin by use of ferulic acid decarboxylase Fdc from Bacillus pumilus and 4-vinylguaiacol oxygenase Cso2 from Caulobacter segnis. In the first stage, Escherichia coli cells expressing Fdc rapidly decarboxylate ferulic acid and completely convert 75 mM of this substrate to 4-vinylguaiacol within 2 h at pH 9.0. In the second stage, Escherichia coli cells expressing Cso2 efficiently oxidize 4-vinylguaiacol to vanillin. The concentration of vanillin reaches 52 mM (7.8 g/l) in 24 h |
734786 |
