EC Number   |
Application |
Reference |
|---|
    3.5.4.1 | agriculture |
CodA can be used as a negative selection marker in Glycine max. Wild-type seedlings are not affected by inclusion of 5-fluorcytosine in growth media, whiule transgenic Glycine max plants expressing CodA and grown in the presence of more than 200 microg/ml 5-fluorocytosine exhibit reductions in hypocotyl and tap-root lengths, and severe suppression of lateral root development |
755034 |
| 3.5.4.1 | agriculture |
construction of a fusion protein of fluorocytosine deaminase FCY with the bacterial uracil phosphoribosyl transferase (UPP) gene. The recombinant protein converts the precursor 5-fluorocytosine into 5-fluorouracyl, used in the treatment of a range of cancers. The FCY-UPP gene construct acts in a cell-autonomous manner and can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. The 5-fluorouracil precursor acts systemically the tissular inactivation is reversible, and can be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages |
-, 755036 |
| 3.5.4.1 | analysis |
crystallization strategy named microseed matrix screening, differential chelation pattern of cations by acidic surfaces of proteins within crystal lattice as a critical parameter of crystal nucleation and growth |
667058 |
| 3.5.4.1 | analysis |
in a selection-rooting assay CodA-expressing aerial tissues or shoot cuttings of Glycine max are inhibited for root formation in media containing 5-fluorouracil |
755034 |
| 3.5.4.1 | biotechnology |
a chitosan-entrapped cytosine deaminase nanocomposite is developped. Sustained release of cytosine deaminase from the nanocomposite up to one week depicts its potential implication in prodrug inducted enzyme therapy |
719155 |
| 3.5.4.1 | diagnostics |
the enzyme, expressed by Clostridium sp., can be used for detection of Clostridia-containing tumors in humans using clostridial directed 5-fluorouracil prodrug therapy |
688802 |
| 3.5.4.1 | medicine |
- |
209497, 209506, 209508 |
| 3.5.4.1 | medicine |
a Salmonella typhimurium strain overexpressing CodA and resistant to 5-fluorouracyl due to deletion of the Upp gene sequence, after infecting tumour cell cultures, shows increased cytotoxic and bystander effects under standard induction conditions. A PurD mutation in the producer strain controls its intracellular proliferation by the presence of adenine and avoids the intrinsic Salmonella cell death induction |
754634 |
| 3.5.4.1 | medicine |
construction of secreted form of enzyme by fusion with pre-prosegment of human tissue plasminogen activator. Secreted enzyme temporarily spares transduced cells and enhanced accumulation of extracellular 5-fluorouracil. Tumors expressing the secreted enzyme have an improved response to 5-fluorocytosine treatment compared to tumors expressing intracellular enzyme |
667211 |
| 3.5.4.1 | medicine |
cytosine deaminase gene therapy combined with radiation treatment in breast carcinoma bone tumor, which is painful and causes osteolysis, is evaluated in a 4T1murine breast carcinoma model, overview |
685937 |
