EC Number   |
Application |
Reference |
|---|
   3.4.24.36 | medicine |
DNA/DNA, DNA/protein and protein/protein based vaccination using gp63 against Leishmania donovani inducing immune responses and conferred protection against challenge infection, humural responses, quantitative overview |
718268 |
| 3.4.24.36 | medicine |
enzyme plays a key role during infection of humans with Leishmania parasites, inhibitors may aid the development of drugs |
-, 653919 |
| 3.4.24.36 | medicine |
parasite virulence is not simply correlated with the activity of enzyme. Enzyme plays a significant role in association with other surface molecules, especially lipophosphoglycan. Overexpression of enzyme can compensate lipophosphoglycan defect in the vertebrate host but in sand flies both molecules fulfill quite different functions |
668690 |
| 3.4.24.36 | medicine |
the endoplasmic reticulum and the Golgi hinderes the exit of GP63 metalloprotease from the parasitophorous vacuole and dampens the cleavage of host proteins by GP63 |
755218 |
| 3.4.24.36 | medicine |
the monitoring of host MMPs levels and GP63 in Leishmania isolated from host samples can be used on the laboratory routine to predict the prognostic and treatment efficacy of American tegumentary leishmaniasis |
754989 |
| 3.4.24.36 | pharmacology |
cationic distearoyl phosphatidylcholine liposomes, used as vaccine adjuvant with the immunodominant 63 kDa glycoprotein of promastigotes, induce significant protection against progressive visceral leishmaniasis in susceptible BALB/c mice. gp63 used without adjuvant elicits partial protection but in association with liposomes exhibits marked resistance in both the livers and spleens of the mice challenged 10 days after the last vaccination. The protective efficacy of liposomal gp63 vaccination is dose dependent, with 2.5 microg of protein showing optimal protection. Mice challenged 12 weeks after immunization are still protected, and a mixed Th1/Th2 response has been induced following immunization |
698241 |
| 3.4.24.36 | pharmacology |
immunization of female BALB/c mice with negatively, positively charged or neutral liposomes encapsulated with rgp63, rgp63 in soluble form. The group of mice immunized with recombinant gp63 encapsulated in neutral liposomes shows a significantly smaller footpad swelling upon challenge with Leishmania major compared with positively or negatively charged liposomes. The mice immunized with neutral liposomes show the lowest splenic parasite burden, the highest IgG2a/IgG1 ratio and IFN-gamma production and the lowest IL-4 level compared to the other groups. The results indicate that a Th1 type of immune response is induced in mice immunized with neutral liposomes more efficiently than positively charged liposomes and conversely negatively charged liposomes induce a Th2 type of immune response |
697817 |
