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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.17.8medicine comparison of 82 ampicillin-resistant Enterococcus faecium isolates from clinical infections. Sequence data reveal the existence of 19 different PLP5 alleles with a difference in 16 amino acid positions spanning from residue 414 to 632. Each allele presents at least five amino acid substitutions (His470/Gln, Asn496/Lys, Ala499/Thr, Glu525/Asp, and Glu629/Val). No correlation between amino acid sequence polymorphism of PBP5 and levels of ampicillin resistance is detected. The levels of PLP5 mRNA expression varies between strains and does not always correlate with levels of ampicillin resistance in clinical isolates 754639
Display the word mapDisplay the reaction diagram Show all sequences 3.4.17.8medicine PBP5 mRNA and protein levels are increased in subclade A1 and A2 ampicillin-resistant strains compared to those in clade B and subclade A2 ampicillin-susceptible strains. There is evidence of major clade-associated rearrangements in the region upstream of PBP5, including large DNA fragment insertions, deletions, and single nucleotide polymorphisms, that may be associated with the differential regulation of PBP5 levels between the Enterococcus faecium clades 752508
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