EC Number   |
Application |
Reference |
|---|
   3.2.2.23 | analysis |
comparison of human Ogg1, Escherichia coli Fpg and endonuclease III for the ability to modify the sensitivity of the comet assay. All three endonucleases recognize oxidative DNA damage and, in addition, Fpg and endonuclease III also recognize alkylation damage. Use of human Ogg1 in the modified comet assay offers a useful alternative to Fpg and is more specific for 8-oxoguanine and methyl-fapy-guanine |
682046 |
| 3.2.2.23 | analysis |
comparison of human Ogg1, Escherichia coli Fpg and endonuclease III for the ability to modify the sensitivity of the comet assay. Use of human Ogg1 in the modified comet assay offers a useful alternative to Fpg and is more specific for 8-oxoguanine and methyl-fapy-guanine |
682046 |
| 3.2.2.23 | analysis |
DNA repair kinetics can be investigated with the comet assay and differences between cell types can be observed |
682052 |
| 3.2.2.23 | analysis |
simple and sensitive FPG activity assay using target-induced self-primed rolling circle amplification and magnetic nanoprobes. A 8-oxoguanine is positioned in duplex DNA containing a P-circle and P1, which together serve as a FPG substrate, rolling circle amplification template, and rolling circle amplification primer probe. The presence of FPG specifically binds and cleaves 8-oxoG containing duplex DNA, resulting in 5'-phosphoryl termini. The rolling circle amplification reaction produces an amount of long tandem-repeat DNA tiles with multiple recognizing regions for the modified DNA probes and biotin-modified DNA probes: A detection limit of 1.033 U/ml for FPG is obtained. FPG assays in human blood serum are also obtained using fluorescence and confocal laser scanning microscopy |
749575 |
| 3.2.2.23 | medicine |
cells from children with Down's syndrome do not display an effective DNA repair after treatment with 10 mM hydrogen peroxide. No difference in the sensitivity to DNA-damaging agents and the efficacy of DNA repair due to age and gender in children with Down's syndrome is observed |
682054 |
| 3.2.2.23 | medicine |
expression of enzyme in human cells from patients belonging to Cockayne syndrome complementation groups A and B completely corrects the repair deficiency in both CS-A and CS-B cells. The sensitivity of CS-B cells to elevated concentrations of potassium bromate is not compensated by expression of enzyme |
679970 |
| 3.2.2.23 | medicine |
expression of enzyme-green fluorescent protein fusion protein in human bladder cells. Cells expressing the fusion protein repair 8-oxoguanine and abasic sites at accelerated rates and are resistant to the oxidizing carcinogen potassium bromate |
680213 |
| 3.2.2.23 | medicine |
lymphocytes of rats exposed to thinner fumes exhibits a significant increase in enzyme-sensitive DNA sites compared with control. The most abundant base oxidation product is 8-oxoguanine, which is the main substrate of enzyme |
682909 |
| 3.2.2.23 | medicine |
melatonin can have a protective effect against oxidative DNA damage by chemical inactivation of a DNA-damaging agent as well as by stimulating DNA repair, but key factors such as DNA-formaidopyrimidine glycosylase, endonuclease III are not affected by melatonin |
682053 |
| 3.2.2.23 | medicine |
no correlation between the S1245C polymorphism of the OGG! Gene and gastric cancer. In contrast, there is a strong correlation between gastric cancer occurrence, impaired DNA repair in human lymphocytes, and the G135C polymorphism of the RAD51 gene |
682051 |
