   3.1.1.67 | synthesis |
comparison of three different fatty acid ethyl ester-producing strains of Saccharomyces cerevisiae. Strain CB2I20, with high expression of a heterologous wax ester synthase gene (ws2) and strain BdJ15, containing disruptions of genes DGA1, LRO1, ARE1, ARE2 and POX1, which prevent the conversion of acyl-CoA to sterol esters, triacylglycerides and the degradation to acetyl-CoA, trigger oxidative stress that consequently influences cellular growth. In strain BdJ15 stress is possibly triggered by disabling the buffering capacity of lipid droplets in encapsulating toxic fatty acids such as oleic acid. There is increased demand for NADPH required for the reduction steps in fatty acid biosynthesis |
750562 |
