EC Number   |
Application |
Reference |
|---|
   3.1.1.110 | synthesis |
construction of a pathway for glycolate production from xylose using xylose dehydrogenase (XdH) and xylonolactonase (xylC). The final engineered strain produces 43.60 g/l glycolate, with 0.91 g/l/h productivity and 0.46 g/g xylose yield |
-, 751483 |
| 3.1.1.110 | synthesis |
for synthesis of D-xylonate in Saccharomyces cerevisiae, coexpression of XylC with D-xylose dehydrogenase XylB facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. The lactone and linear forms of D-xylonic acid are produced, accumulate intracellularly, and are partially exported within 15-60 min of D-xylose provision |
-, 754622 |
| 3.1.1.110 | synthesis |
production of xylonate from xylose in Escerichia coli. Through the coexpression of a xylose dehydrogenase (XdH) and a xylonolactonase (XylC) from Caulobacter crescentus, the recombinant strain can convert 1 g/l xylose to 0.84 g/l xylonate and 0.10 g/l xylonolactone. After disruption of endogenous genes XylA and XylB encoding xylose isomerase and xylulose kinase, the finally engineered strain under fed-batch conditions, produces up to 27.3 g/l xylonate and 1.7 g/l xylonolactone from 30 g/l xylose, about 88% of the theoretical yield |
-, 755195 |
| 3.1.1.110 | synthesis |
when engineering Saccharomyces cerevisiae for production of D-xylonate from D-xylose, a strain expressing the D-xylonolactone lactonase xylC along with D-xylose dehydrogenase xylB excretes more D-xylonate earlier during the cultivation, both at pH 5.5 and at pH3, than the strains lacking the lactonase. In Sacchaormyces cerevisiae, the linear form may be more toxic than the lactone form and a more gradual hydrolysis of the lactone form may be advantageous to the cell |
-, 754621 |
