EC Number   |
Application |
Reference |
|---|
    2.5.1.56 | synthesis |
construction of a recombinant Escherichia coli simultaneously overexpressing GlcNAc 2-epimerase and N-acetylneuraminic acid synthase. The recombinant strain produces 25 g/l N-acetylneuraminic acid in 22 h without the addition of Corynebacterium ammoniagenes cells. For manufacturing on an industrial scale, it is preferable to use unconcentrated culture broth as the source of enzymes. The N-acetylneuraminic acid aldolase gene of the host strain is disrupted yielding a strain which cannot degrade N-acetylneuraminic acid. After a 22 h reaction with 120 g/l N-acetylglucosamine in a 5 l jar fermenter, the culture broth of contains 53 g/l N-acetylneuraminic acid |
721977 |
| 2.5.1.56 | synthesis |
design of an in vivo NeuAc biosensor and application for genetic screening of a mutant library of NeuAc producers. A Ni2+-based selection system couples the cell growth with in vivo NeuAc concentration. The final strain produces up to 8.31 g/l NeuAc in minimal medium using glucose as sole carbon source |
-, 759716 |
| 2.5.1.56 | synthesis |
development of a novel microbial transformation for the synthesis of N-acetyl-D-neuraminic acid using bacterial cells expressing N-acetyl-D-glucosamine 2-epimerase and N-acetylneuraminate synthase |
637322 |
| 2.5.1.56 | synthesis |
NeuAc production in Bacillus subtilis is enhanced to 46.04 g/l, by expressing NanA from Staphylococcus hominis. Enhanced expression of NanAand blocking formation of the by-product acetoin from pyruvate, result in 68.75 g/l NeuAc production with a molar conversion rate of 55.57% from GlcNAc |
758954 |
| 2.5.1.56 | synthesis |
the enzyme might be useful for production of sialic acids and derivates at low temperatures |
737983 |
