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2.4.1.79
synthesis
synthesis of globoside and isogloboside tetrasaccharides by using beta(1->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein
658435
2.4.1.79
synthesis
synthesis of globotetraose (GalNAcb-3Gala-4Galb-4Glc)by the high cell density culture of an Escherichia coli strain over-expressing the Neisseria meningitidis lgtC gene for alpha-1,4-Gal transferase. The strain which is devoid of both alpha and beta galactosidase activity is fed with glycerol as the energy and carbon source and with lactose as precursor for globotriose synthesis. After complete exhaustion of lactose, globotriose could serve as an alternative acceptor for LgtC and the formation of a series of polygalactosylated compounds is observed. The system is extended to the synthesis of globotetraose (GalNAcbeta-3Gala-4Galbeta-4Glc) by overexpressing two additional genes: lgtD from Haemophilus influenzae Rd which encodes a beta-1,3-GalNAc transferase and wbpP from Pseudomonas aeruginosa which encodes a UDP-GalNAc C4 epimerase. Globotetraose could also be produced from exogenous globotriose which is actively taken up by the cells
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