EC Number   |
Application |
Reference |
|---|
   2.4.1.34 | agriculture |
silencing of enzyme genes GSL5, GSL6, GSL11 with RNAi: both wound callose and papillary callose are absent in lines transformed with GSL5 dsRNAi, but unaffected in GSL6 and GSL11 RNAi lines. Absence of callose in palpillae or haustorial complexes correlates with effective growth cessation of several normally virulent powdery mildew species and of Peronospora parasitica |
660125 |
| 2.4.1.34 | analysis |
glucan synthase activity assay based on size-exclusion chromatography coupled with pulsed-amperometric detection and radiation counting (SEC-PAD-RC), which allows for the simultaneous characterization of the amount and length of the polymer product |
-, 756068 |
| 2.4.1.34 | analysis |
simple and sensitive method for characterization of enzyme products by analysis of newly synthesized polysaccharides by 13C-nuclear magnetic resonance |
658851 |
| 2.4.1.34 | medicine |
exposure of strain RG101 to caspofungin during growth yields a modified enzyme that is drug insensitive (4 log orders) in kinetic inhibition assays, and this insensitivity is also observed for enzymes isolated from clinical isolates. The lipid microenvironment of the enzyme with resistance induced by caspofungin reveals a prominent increase in the abundances of dihydrosphingosine and phytosphingosine. Exogenous addition of dihydrosphingosine and phytosphingosine to the sensitive enzyme recapitulates the drug insensitivity of the caspofungin-derived enzyme. Caspofungin induces mitochondrion-derived reactive oxygen species, and dampening reactive oxygen species formation by antimycin A or thiourea eliminates drug-induced resistance |
-, 757549 |
