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2.4.1.265
synthesis
expression of the genes encoding for alginate-lyase algL and the catalytic subunit of the alginate polymerase complex, dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase alg8, in chemostat cultures of Azotobacter vinelandii ATCC 9046. Increased alginate production of 2.4 g per l and a higher specific alginate production rate of 0.1 g per g and h are obtained at an inlet sucrose concentration of 15 g per l. Carbon recovery of about 100% is obtained at an inlet sucrose concentration of 10 g per l, whereas it is close to 50% at higher inlet sucrose concentrations. An increase in the molecular weight of alginate is linked to higher alg8 gene expression
722890
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