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2.3.1.301
analysis
discontinuous assay for the FabH enzyme that eliminates the need for the washing steps or specialty scintillation proximity assay beads and the preparation of acyl carrier proteins. The assay involves the reduction of radiolabled long-chain beta-ketoacyl CoA product to its dihydroxy derivative, which partitions into a nonpolar phase for quantitation, while the reduced radiolabeled substrate derivative remains in the aqueous phase
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761827
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