  2.3.1.229 | synthesis |
development of an artificial biosynthetic process for phenylacetyl-homoserine lactone analogs, including cinnamoyl-homoserine lactone, 4-coumaroyl-homoserine lactone, caffeoyl-homoserine lactone, and feruloyl-homoserine lactone, using coexpression of the codon-optimized synthase RpaI and 4-coumaroyl-CoA ligase 4CL2nt, EC 6.2.1.12, in Escherichia coli. De novo production of p-coumaroyl-homoserine lactone in Escherichia coli can be achieved by expression of the rpaI gene in addition to 4-coumaroyl-CoA biosynthetic genes. The yields for 4-coumaroyl-homoserine lactone reach 93 and 142 mg/l in the S-adenosyl-L-methionine and L-methionine feeding culture, respectively |
736751 |
