EC Number   |
Application |
Reference |
|---|
   2.3.1.211 | analysis |
engineering of an in vivo reporter assay for phenylalanine ammonia-lyase efficiency in Escherichia coli based on a plant type III polyketide biosynthetic pathway. The candidate phenylalanine ammonia-lyases are coexpressed with 4-coumarate:CoA ligase 4CL1 from Arabidopsis thaliana and curcuminoid synthase from Oryza sativa. A microplate-based assay is used to measure the titer of dicinnamoylmethane |
735539 |
| 2.3.1.211 | synthesis |
an artificial pathway for curcuminoid production in Escherichia coli is constructed. Overexpression of curcuminoid synthase from Oryza sativa in Escherichia coli results in the production of the major curcuminoid, bisdemethoxycurcumin from p-coumaric acid. It is demonstrated that enhancement of the intracellular malonyl-CoA pool is essential for increasing the final production titer of bisdemethoxycurcumin. Expression of a recombinant pathway that allows the conversion of malonate to malonyl-CoA encoded by genes matB and matC results in a 25fold improvement of final bisdemethoxycurcumin titer |
756259 |
| 2.3.1.211 | synthesis |
an engineered Escherichia coli strain expressing phenylalanine ammonia-lyase, 4-coumarate:CoA ligase 4CL1 from Arabidopsis thaliana and curcuminoid synthase from Oryza sativa leads to the production of dicinnamoylmethane at a high level of 0.36 g/l. Supplement of 2-fluoro-phenylalanine yields fluorinated dicinnamoylmethane derivatives, 6,6'-difluorodicinnamoylmethane and 6-fluoro-dicinnamoylmethane, of which the latter is a new curcuminoid |
735539 |
| 2.3.1.211 | synthesis |
engineering of a Pseudomonas putida strain to produce bisdemethoxycurcumin |
757576 |
| 2.3.1.211 | synthesis |
production of curcuminoids in engineered Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l |
757419 |
| 2.3.1.211 | synthesis |
production of curcuminoids in engineered Escherichia coli. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) are synthesized from glucose in Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l |
757419 |
