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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.140synthesis expression of rosmarinic acid synthase from Coleus blumei, 4-coumarate: CoA ligase from Arabidopsis thaliana, 4-hydroxyphenyllactate 3-hydroxylase from Escherichia coli and D-lactate dehydrogenase from Lactobacillus pentosus in an L-tyrosine over-producing Escherichia coli strain leads to a yield of rosmarinic acid of 130 mg/l. In addition, intermediate caffeoyl-phenyllactate is produced at about 55 mg/l 756267
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.140synthesis production of rosmarinic acid analogues in Escherichia coli by expression of 4-coumarate: CoA ligase from Arabidopsis thaliana and rosmarinic acid synthase from Coleus blumei. Incubation of the recombinant strain with exogenously supplied phenyllactic acid and analogues, and coumaric acid and analogues as donor substrates leads to production of 18 compounds, including unnatural analogues 756266
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