EC Number   |
Application |
Reference |
|---|
   2.1.1.34 | analysis |
Arg-41 is the catalytic center, Lys-90, Arg-166, Arg-168, and Arg-176 are the initial sites of tRNA binding, Arg-8, Arg-19, and Lys-32 are required in the tRNA binding site for continuation the catalytic cycle, Arg-11-His-71-Met-147 interaction is involved in the structural element in release of S-adenosyl-L-homocysteine, His-34 plays a role in the assisted phosphate binding site, Arg-109 has an unknown function |
674692 |
| 2.1.1.34 | analysis |
dissociation of tRNA from the tRNA-enzyme complex after the methyl-transfer reaction is caused by the dissociation of enzyme subunits |
-, 676232 |
| 2.1.1.34 | analysis |
reveals a fold typical of members of the SpoU clan of proteins, a subfamily of the alpha/beta-knot superfamily, with alpha-helical extensions at the N- and C-termini that are likely to be involved in tRNA binding |
671082 |
| 2.1.1.34 | analysis |
through the log phase, the content of the TrmH protein is not changed obviously, but in the stationary phase, the content of the TrmH protein is slowly reduced |
-, 676234 |
| 2.1.1.34 | medicine |
an RNA preparation of an Escherichia coli trmH mutant that lacks Gm18 2'-O-methyltransferase activity is significantly more immunostimulatory in human peripheral blood mononuclear cells than the wild-type sample. The single methyl group on the 2'-oxygen of Gm18 is a natural modification in native tRNA that, beyond its primary structural role, has acquired a secondary function as an antagonist of toll-like receptor TLR7 |
734391 |
| 2.1.1.34 | medicine |
bacterial tRNA induces type I interferon and inflammatory cytokines in mouse dendritic cells and human peripheral blood mononuclear cells. tRNA from an Escherichia coli knockout strain for tRNA (Gm18)-2'-O-methyltransferase regained immunostimulatory potential. In vitro methylation of this immunostimulatory Gm18-negative tRNA with recombinant trmH from Thermus thermophilus abolishes its interferon-alpha-inducing potential. Gm18-modified tRNA acts as toll-like receptor TLR7 antagonist and blocks IFN-alpha induction of influenza A virus-infected peripheral blood mononuclear cells |
734392 |
| 2.1.1.34 | medicine |
in vitro methylation of immunostimulatory Gm18-negative tRNA with recombinant trmH from Thermus thermophilus abolishes its interferon-alpha-inducing potential |
734392 |
