EC Number   |
Application |
Reference |
|---|
    1.4.1.9 | analysis |
assay of serum and urine for urea with use of urease and leucine dehydrogenase |
349698 |
| 1.4.1.9 | analysis |
cheap and rapid determination of branched-chain amino and oxo acids |
349697 |
| 1.4.1.9 | analysis |
flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase |
349678 |
| 1.4.1.9 | analysis |
high-performance liquid chromatographic determination of branched-chain alpha-keto acids in serum using immobilized leucine dehydrogenase as post-column reactor |
349699 |
| 1.4.1.9 | analysis |
postcolumn co-immobilized leucine dehydrogenase-NADH oxidase reactor for the determination of branched-chain amino acids by high-performance liquid chromatography with chemiluminescence detection |
349689 |
| 1.4.1.9 | biotechnology |
an efficient stereospecific enzymatic synthesis of L-valine, L-leucine, L-norvaline, L-norleucine and L-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1-10 g) |
693504 |
| 1.4.1.9 | drug development |
construction of bifunctional formate dehydrogenase and leucine dehydrogenase enzymatic complex for efficient cofactor regeneration and L-tert leucine biotransformation. L-tert leucine is a widely used chiral building block in many asymmetric reactions for the synthesis of anti-tumor and anti-HIV drugs |
-, 742376 |
| 1.4.1.9 | synthesis |
coexpression with Bacillus megaterium glucose dehydrogenase in Escherichia coli for the production of L-tert-leucine. A decagram preparation of L-tert-leucine is performed at a substrate concentration of 0.6 M in 1 l scale with 99% conversion after 5.5 h, resulting in 80.1% yield and > 99% enantiomeric excess |
743093 |
| 1.4.1.9 | synthesis |
coexpression with NAD+-dependent FDH from Candida boidinii in Escherichia coli for synthesis of L-tert-leucine. In a continuous feeding process, at an overall substrate concentration up to 1.5 M, both conversion and enantiomeric excess of >99% and space-time yield of 786 g/l/d are achieved |
-, 741837 |
| 1.4.1.9 | synthesis |
conversion of ammonia or urea into essential amino acids, L-Leu, L-Val, and L-Ile, using artificial cells containing an immobilized multienzyme system that consists of EC 1.1.1.1, EC 1.4.1.9, EC 3.5.1.5 and dextran-NAD+ |
349696 |
