1.3.1.109 | synthesis |
engineering of Clostridum sp. MT1962 by elimination of phosphotransacetylase and acetaldehyde dehydrogenase along with integration to chromosome synthetic thiolase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, butyryl-CoA dehydrogenase, butyraldehyde dehydrogenase, and NAD-dependent butanol dehydrogenase. Th resulting strain loses production of ethanol and acetate while initiated the production of 297 mM of n-butanol |
-, 741631 |