EC Number   |
Application |
Reference |
|---|
   1.2.3.3 | analysis |
biosensor for inorganic phosphate |
671504 |
| 1.2.3.3 | analysis |
construction of an amperometric biosensor using nanoparticles of pyruvate oxidase, immobilized covalently onto pencil graphite electrode. The biosensor shows ideal working within 5 s under defined conditions of pH 6.0 and 30°C at an applied voltage of -0.1 V. Under standard assay conditions, a linear response is obtained between pyruvate concentration from 0.001 to 6000 microM and current. Lower detection limit is 0.58 microM, the biosensor can be used for over 210 days for the measurement of pyruvate in blood sera |
763660 |
| 1.2.3.3 | analysis |
rapid detection of phosphate using immobilized pyruvate oxidase |
390431 |
| 1.2.3.3 | analysis |
use of nanoparticles of commercially available pyruvate oxidase covalently immobilized onto gold electrode as amperometric pyruvate biosensor for detection of pyruvate in serum. The biosensor shows optimum response within 7.5 s, at a potential of 0.28 V, pH 5.5 and 35°C and a lower detection limit of 0.67 microM. The analytical recovery of added pyruvate in sera is 99.0% and 99.5% within and between batches. The biosensor can be utilized for detection of total pyruvate level in sera of apparently healthy individuals and patients suffering from cardiogenic stress |
762983 |
| 1.2.3.3 | medicine |
Lactobacillus delbrueckii commercia l yogurt strains STYM1 and GVKM1 inhibit Pseudomonas gingivalis growth in vitro, while raw milk isolates SYB7 and SYB13 have little impaxct. Purified, catalytically active, recombinant pyruvate oxidase is sufficient to inhibit P. gingivalis growth in vitro without the addition of cofactors |
762645 |
| 1.2.3.3 | more |
aerobiosis makes the concerted action of lactate oxidase and pyruvate oxidase possible, enabling cells of Streptococcus pneumoniae to gain more ATP from glucose than under anaerobiosis |
-, 687414 |
| 1.2.3.3 | more |
development of a biosensor based on POX enzyme for the investigation of the effect of thiamine (vitamin B1) molecule on the activity of the enzyme |
685699 |
| 1.2.3.3 | more |
optimization of the medium for PyOD production by recombinant Escherichia coli using shake flask method |
-, 693477 |
| 1.2.3.3 | more |
pyruvate oxidase is not only a stationary-phase enzyme. Removal of the poxB gene affects the central metabolism at the enzyme level |
695267 |
| 1.2.3.3 | synthesis |
constitutive overproduction of enzyme, acetate becomes the only fermentation end product |
-, 655878 |
