EC Number   |
Application |
Reference |
|---|
   1.14.99.53 | analysis |
development of a chitinase-coupled assay method and an assay based on consumption of ascorbate. There is no H2O2 production in the presence of colloidal chitin at 4 mg/ml |
-, 740398 |
| 1.14.99.53 | analysis |
method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity |
740448 |
| 1.14.99.53 | degradation |
presence of lytic polysaccharide monooxygenase CBP21 facilitates the degradation of chitin substrates (colloidal chitin, beta-chitin, and alpha-chitin) by Chi92 |
743999 |
| 1.14.99.53 | synthesis |
construction of a cassette vector containing the XylS/Pm system that can easily be used for exchanging LPMO coding genes with or without signal sequences. The cassette reliably produces mature (translocated) LPMOs under controlled conditions. The signal sequence of LPMO10A from Serratia marcescens gives highest levels of recombinant protein production and translocation |
744631 |
| 1.14.99.53 | synthesis |
efficient production in Escherichia coli is achieved using PelB as the most productive signal peptide for the extracellular production of CBP21 and Aeromonas veronii B565 chitinase Chi92 |
743999 |
