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EC Number	Application	Commentary	Reference
1.14.20.6	analysis	an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods	742549
1.14.20.6	synthesis	functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin	742957
1.14.20.6	synthesis	production of active flavonol synthase from Camellia sinensis in Escherichia coli, with high specific activity	689865

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Release 2023.1 (January 2023)