EC Number   |
Application |
Reference |
|---|
    1.14.13.1 | analysis |
an amperometric biosensor is developed for the interference-free determination of L-glutamate with a bienzyme-based Clark electrode. This sensor is based on the specific dehydrogenation by L-glutamate dehydrogenase (EC 1.4.1.3) in combination with salicylate hydroxylase (EC 1.14.13.1). The enzymes are entrapped by a poly(carbamoyl) sulfonate (PCS) hydrogel on a Teflon membrane |
686457 |
| 1.14.13.1 | analysis |
biosensor system for determining salicylate in body fluids |
390016 |
| 1.14.13.1 | analysis |
two types of amperometric ATP biosensors are developed by using the coimmobilization of salicylate hydroxylase (SHL, EC 1.14.13.1), glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), and hexokinase (HEX, EC 2.7.1.1) on a Clark-type oxygen electrode and on a screenprinted electrode. The principles of the determination schemes are as follows: HEX transfers the phosphate group from ATP to glucose to form glucose-6-phosphate. G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by consuming NAD+. The product, NADH initiates the irreversible decarboxylation and hydroxylation of salicylate by SHL to consume dissolved oxygen and generate catechol. This results in a detectable signal on a Clark-type electrode due to the SHL-enzymatic consumption of oxygen, or a detectable signal on a screen-printed electrode due to the SHL-enzymatic generation of catechol in the measurement of ATP. Both sensors show high performance characteristics with broad detection ranges, short measuring times, and good specificities |
690072 |
| 1.14.13.1 | analysis |
usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses |
710856 |
| 1.14.13.1 | diagnostics |
usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses |
710856 |
