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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.13molecular biology a multicolor BRET assay for the quantification of global DNA methylation is developed using CXXC-fused Oplophorus luciferase (CXXC-Oluc), a methyl-CpG-binding domain-fused firefly luciferase (MBD-Fluc), BOBO-1, and BOBO-3. CXXC-Oluc recognized unmethylated CpG sites on genomic DNA to excite BOBO-1 DNA intercalating dye, whereas MBD-Fluc recognized methylated CpG sites on genomic DNA to excite BOBO-3 DNA intercalating dye. The emission intensities of BOBO-1 and BOBO-3 are simultaneously detected and depended on the unmethylated and methylated CpG contents of the genomic DNA. There is a significant negative correlation between the emission intensities of BOBO-1 and BOBO-3. Therefore, the global DNA methylation level can be quantified with this multicolor BRET assay using a single tube 763876
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.13molecular biology bioluminescence imaging is a powerful, broadly utilized method for non-invasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies of protein-protein interactions, ligand-receptor interactions, cell trafficking, and drug targeting in mouse models. The use of a luciferase enzyme derived from Oplophorus gracilirostris, NanoLuc, is described in cell-based assays bioluminescence imaging of tumor-bearing mice. NanoLuc is combined with another luciferase enzyme, firefly luciferase, to image multiple signal transduction events in one imaging session 765340
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.13molecular biology useful reporter protein in various assay systems including reporter assays and immunoassays 696008
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