EC Number   |
Application |
Reference |
|---|
   1.1.1.50 | analysis |
engineering of 3alpha-hydrosteroid biosensor for androsterone determination by immobilizing the enzyme 3alpha-hydroxysteroid dehydrogenase in a composite electrode platform constituted of a mixture of multi-walled carbon nanotubes, octylpyridiniumhexafluorophosphate ionic liquid and NAD+ cofactor.This configuration allows the fast, sensitive and stable electrochemical detection of the NADH generated in the enzyme reaction. Reaction is linear for androsterone in the 0.510 microM concentration range. The detection limit achieved is 0.15 microM |
726505 |
| 1.1.1.50 | analysis |
replacement of hsdA gene by the green fluorescent protein gene inserted downstream from the hsdA regulatory region and use of the resulting strain as fluorescence based biosensor system for steroid determination. With this cell-based system, testosterone can be determined in a range between 57 and 450 ng/ml, estradiol between 1.6 and 12.8 ng/ml, and cholesterol between 19.3 and 154.4 ng/nl. The sensitivity of this bioassay can be further increased by using only the cytosol of the mutant. With the resulting cell-free system testosterone is determined in a range between 28 and 219 pg/ml, estradiol between 0.029 and 0.430 fg/ml, and cholesterol between 9.7 and 77.2 fg/ml. The recovery ratio of the extraction is around 95% and the maximum fluorescence signals are obtained as early as after 30 min. Limitations of the established steroid biosensor system are quenching at higher steroid concentrations and the relatively high background of fluorescence |
697270 |
| 1.1.1.50 | biotechnology |
transfection of cells with plasmids encoding a 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion protein. The Del1 deposition domain immobilizes the enzyme in the extracellular matrix without interfering with its enzymatic activity. Extracellular matrix conditioned by cells transfected with 3alpha-hydroxysteroid dehydrogenase-Del1 deposition domain fusion significantly suppresses the growth of otherwise untreated LNCaP cells |
696786 |
| 1.1.1.50 | drug development |
the enzyme is a target for treatment of chronic pain in neuropathic diseases, overview |
689169 |
| 1.1.1.50 | environmental protection |
the mutant Comamonas testosteroni strain CT-GFP5-1 can be used as a sensitive biosensor system for steroid determination in the environment |
697270 |
| 1.1.1.50 | medicine |
enzyme as part of commercial enzyme test kit for bile acid content in serum as an index for hepatic diseases |
389552 |
| 1.1.1.50 | medicine |
enzyme is a potential drug target |
655214 |
| 1.1.1.50 | synthesis |
the enzyme is useful foe androsterone production in a coupled system with formate dehydrogenase in enhancing Tris-HCl/co-solvent 1-butyl-3-methylimidazolium L-lactate, at pH 7.6 and 25°C, method optimization, overview |
674981 |
| 1.1.1.50 | synthesis |
the enzyme is useful in reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration |
674981 |
