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EC Number Application Commentary Reference
Show all pathways known for 1.1.1.365Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.365nutrition accumulation of high levels of ascorbate using GalUR gene overepression in tomato fruits. Tomato fruits are considered a major dietary source of vitamin C in many countries, because it is consumed regularly and in large quantities. Tomato also serves as a fruit model for other crops species with fleshy berry. Accordingly, it is very important to monitor and to increase the vitamin C content in tomato fruit, for meeting the consumer demand and health requirements for high nutrition, e.g. by overexpressing D-galacturonate reductase 743465
Show all pathways known for 1.1.1.365Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.365synthesis L-galactonate shows potential applications as an additive to nutrients and cosmetics. AnGar1 variants are developed that represent the a GalA reductases with a higher preference for NADH compared to NADPH. The altered cofactor-specificity enables the coupling of GalA reduction to glycolysis, resulting in higher yields of GalOA when glucose is used as a redox donor. The engineered AnGar1 should prove valuable for D-galacturonic acid utilization in pectin-rich hydrolysates, which contain neutral sugars such as glucose, galactose, or arabinose, all of which are funneled into glycolysis. The NADH-dependent GalA reductases can facilitate the coupling of L-galactonate production to the oxidation of glycerol, an abundant waste product that can be supplemented to pectin-rich hydrolysates -, 763698
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