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Results 1 - 6 of 6
EC Number
Application
Commentary
Reference
analysis
96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity by biotinylation of the polypeptide p67, which comprises the 67 C-terminal amino acids in human propionyl-CoA carboxylase, including the biotin-binding site lysine-669, using IRDye-streptavidin and infrared spectroscopy. The minimal concentration of recombinant HCS that can be detected by this assay is less than 1.08 nmol/l. Jurkat cells contain 0.14 U of HCS activity [in micromol of biotinylated p67 formed/(nmol/l HCS h)] in 400 microg of total protein
analysis
fusion of full-length HCS to DNA adenine methyltransferase Dam and subsequent transfection into breast cancer cells MCF-7 and normal breast cells MCF-10A for identification of chromatin binding sites
diagnostics
the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
medicine
HCS deficiency results in biotin-responsive multiple carboxylase deficiency
medicine
identifaction of patients with holocarboxylase synthetase deficiency by newborn screening
synthesis
site-directed antibody immobilization by fusing biotin carboxyl carrier protein BCCP to the IgG-binding domain of Protein A, and the resulting fusion protein is immobilized on the biotin protein ligase-modified gold surface of the sensor chip for quartz crystal microbalance through complexation between BCCP and biotin protein ligase. The layer of the IgG-binding domain is successfully captured the antibody, and the antibody retains high antigen-binding capability
Results 1 - 6 of 6