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development of a high-throughput screening method to discriminate between different alginate structures
in vitro assay for mannuronan C5 epimerization wherein extracts of Escherichia coli expressing high levels of AlgG are incubated with polymannuronate. Epimerization of D-mannuronate to L-guluronate residues in the polymer is detected enzymatically, using a L-guluronate-specific alginate lyase of Klebsieila aerogenes
rapid and reproducible assay for the analysis of epimerase activity in the fermentation broth using [3H]-alginate as a substrate. The effects of potential interfering compounds are characterized and the uncertainty of the analysis system has been determined. A standard method suitable for use directly on the fermentation broth with a standard deviation within a single analysis of 2.5% has been developed
Results 1 - 3 of 3